Overexpression of genes encoding enzymes involved in trehalose synthesis from Caragana korshinskii enhances drought tolerance of transgenic plants

Trehalose, which plays important roles in resistance to abiotic stresses and preservation of biological activity plants, is synthesized by two key enzymes, trehalose-6-phosphate synthase (TPS) phosphatase (TPP). Therefore, the expressions TPS TPP genes directly affect trehalose synthesis stress plants. In this study, CkTPS CkTPP from Caragana korshinskii were identified, role adaptation desert plant adverse conditions was investigated. Higher observed roots, whereas much lower leaves stems, their upregulated under drought stress. Histochemical analyses showed that β-glucuronidase expression driven promoters strongly induced phytohormones, such as abscisic acid, gibberellin, methyl jasmonate, mannitol, suggests may be regulated various signaling pathways. To determine functional mechanism underlying regulating response introduced into Arabidopsis. Compared wild-type (WT) these transgenic plants higher germination rate, survival, less damage, better shoot growth, longer roots Moreover, had a significantly content proline, chlorophyll, trehalose, activities antioxidant including superoxide dismutase (SOD), peroxidase (POD) catalase (CAT), malondialdehyde (MDA) than WT controls. Double-transgenic carrying growth stronger tolerance either single line. These results provide theoretical experimental basis for further understanding function regulatory CkTPP, well possibility application improving crops through genetic engineering.